Patricia Carla de Oliveira, Fernando Henrique Barbosa da Silva, Cátia Nunes da Cunha


Research reports have suggested that the soil seed banks can have seedling recruitment favoured by fire, especially in grassy plant communities prone to this environmental factor. We examine effects of fire on the soil seed bank of grasslands in the Neotropical wetland Pantanal (hyperseasonal savanna). Our hypothesis was of negative effect, given the contrasting traits associated to seed-seedling tolerance to fire or flooding in addition to the strength of flood as the main ecological driver in floodable areas. Soil samples were collected before and immediately after a prescribed burn in floodable savanna grasslands, at different depths (2 cm layers down to the 10 cm depth), plus litter. The soil samples were taken to a greenhouse for censuses of seedling emergence, to assess the species composition of the seed bank. We recorded a density of 7404 seeds/m2 and 49 morphospecies, mostly aquatic plants (63%). Among the analysed ecological parameters, the species richness and composition did not change significantly between the pre and postfire conditions; yet the abundance was significantly lower only in the first 2 cm layer of the burned soil. Both abundance and richness decrease with soil depth. This apparent fire tolerance of the soil seed bank of floodable savanna grasslands is here proposed as a clue to understand fire as another ecological driver, as well as flood, but further long-term studies are needed.


aquatic macrophytes; plant ecology; prescribed fire; reproductive strategy; soil depth

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Alvares, C. A., Stape, J. L., Sentelhas, P. C., Gonçalves, J. L. M., & Sparovek, G. 2013. Köppen’s climate classification map for Brazil. Meteorologische Zeitschrift, 22, 711–728. DOI: 10.1127/0941-2948/2013/0507

Assine, M. L., & Soares, P.C. 2004. Quaternary of the Pantanal, west-central Brazil. Quaternary International, 114, 23–34. DOI: 10.1016/S1040-6182(03)00039-9

Bao, F., Pott, A., Ferreira, F. A., & Arruda, R. 2014. Soil seed bank of floodable native and cultivated grassland in the Pantanal wetland: effects of flood gradient, season and species invasion. Brazilian Journal of Botany, 7(3), 239–250. DOI: 10.1007/s40415-014-0076-z

Baskin, C. C., & Baskin, J. M. 2001. Seeds: ecology, biogeography, and evolution of dormancy and germination. San Diego: Academic Press: p. 666.

Brooks, M. L., D’Antonio, C. M., Richardson, D. M., Grace, J. B., Keeley, J. E., Ditomaso, J. M., Hobbs, R. J., Pellant, M., & Pyke, D. 2004. Effects of invasive alien plants on fire regimes. BioScience, 54(7), 677–688.

Cardoso, E. L., Crispim, S. M. A., Rodrigues, C. A. G., & Barioni-Junior, W. 2000. Biomassa aérea e produção primária do estrato herbáceo em campo de Elyonurus muticus submetido à queima anual, no Pantanal. Pesquisa Agropecuária Brasileira, 35(8), 1501–1507. DOI: 10.1590/S0100-204X2000000800001

Cardoso, E. L., Crispim, S. M. A., Rodrigues, C. A. G., & Barioni Junior, W. 2003. Efeitos da queima na dinâmica da biomassa aérea de um campo nativo no Pantanal. Pesquisa Agropecuária Brasileira, 38(6), 747–752. DOI: 10.1590/S0100-204X2003000600011

Cochrane, M. A. 2003. Fire Science for rainforests. Nature, 421(27), 913–919. DOI: 10.1038/nature01437

Costa, C. P., Nunes-da-Cunha, C., & Costa, S. C. 2010. Caracterização da flora e estrutura do estrato arbustivo-arbóreo de um cerrado no Pantanal de Poconé, MT. Biota Neotropica, 10, 61–73. DOI: 10.1590/S1676-06032010000300006

Coutinho, L. M. 1990. Fire in the Ecology of the Brazilian Cerrado. In: J. G. Goldammer (Ed.). Fire in the Tropical Biota - Ecological Studies. pp. 82-105. Berlin: Springer-Verlag.

Dawson, S. K., Kingsford, R. T., Berney, P., Keith, D. A., Hemmings, F. A., Warton, D. I., Waters, C., & Catford, J. A. 2016. Frequent inundation helps counteract land use impacts on wetland propagule banks. Applied Vegetation Science, 20, 459–467. DOI: 10.1111/avsc.12295

Fenner, M. & Thompson, K. 2005. Soil Seed Banks. In: M. Fenner, & K. Thompson. 2005. The Ecology of Seeds. pp. 76–96. Cambridge: Cambridge University Press.

Fichino, B.S., Dombroski, J. R. G., Pivello, V. R., & Fidelis, A. 2016. Does fire trigger seed germination in the Neotropical savannas? Experimental tests with six Cerrado species. Biotropica, 48(2), 181–187. DOI: 10.1111/btp.12276

Flora do Brasil 2020 em construção. Rio de Janeiro Botanical Garden. Retrieved on 12 August, 2019, from http://floradobrasil.jbrj.gov.br/

Hölzel, N., & Otte, A. 2001. The impact of flooding regime on the soil seed bank of flood-meadows. Journal of Vegetation Science, 12, 209–218. DOI: 10.2307/3236605

Ikeda, F. S., Mitja, D., Vilela, L., & Silva, J. C. S. 2008. Banco de sementes em cerrado sensu stricto sob queimada e sistemas de cultivo. Pesquisa Agropecuária Brasileira, 43(6), 667–673. DOI: 10.1590/S0100-204X2008000600001

Izhaki, I., Henig-Sever, N., & Ne’eman, G. 2000. Soil seed banks in Mediterranean Aleppo pine forests: the effect of heat, cover and ash on seedling emergence. Journal of Ecology, 88, 667–675. DOI: 10.1046/j.1365-2745.2000.00486.x

Jackson, M. B., & Colmer, T. D. 2005. Response and adaptation by plants to flooding stress. Annals of Botany, 96(4), 501–505. DOI: 10.1093/aob/mci205

Jalili, A., Hamzeh’ee, B., Asri, Y., Shirvany, A., Yazdani, S., Khoshnevis, M., Zarrinkamar, F., Ghahramani, M-A., Safavi, R., Shaw, S., Hodgson, J. G., Thompson, K., Akbarzadeh, M., & Pakparvar, M. 2003. Soil seed banks in the Arasbaran Protected Area of Iran and their significance for conservation management. Biological Conservation, 109, 425–431. DOI: 10.1016/s0006-3207(02)00170-2

Jankowska-Błaszczuk, M., & Grubb, P. J. 2006. Changing perspectives on the role of soil seed bank in northern temperate deciduous forests and in tropical lowland rain forests: parallels and contrasts. Perspectives in Plant Ecology, Evolution and Systematics, 8, 3–21. DOI: 10.1016/j.ppees.2006.06.001

Junk, W. J., Bayley, P. B., & Sparks, R. E. 1989. The flood pulse concept in river-floodplain systems. Canadian Special Publication of Fisheries and Aquatic Sciences, 106, 100–127.

Junk, W. J., Nunes da Cunha, C., Wantzen, K. M., Petermann, P., Strüssmann, C., Marques, M. I., & Adis, J. 2006. Biodiversity and its conservation in the Pantanal of Mato Grosso, Brazil. Aquatic Sciences, 68, 278–309. DOI: 10.1007/s00027-006-0851-4

Junk, W. J., & Nunes-da-Cunha, C. 2012. Pasture clearing from invasive woody plants in the Pantanal: a tool for sustainable management or environmental destruction? Wetlands Ecology and Management, 20, 111–122. DOI: 10.1007/s11273-011-9246-y

Junk, W. J., Piedade, M. T. F., Lourival, R., Wittmann, F., Kandus, P., Lacerda, L. D., Bozelli, R. L., Esteves, F. A., Nunes-da-Cunha, C., Maltchik, L., Schöngart, J., Schaeffer-Novelli, Y., Agostinho, A. A., Nobrega, R. L. B., & Camargo, E. 2015. Definição e classificação das áreas úmidas (AUs) brasileiras: Base científica para uma nova política de proteção e manejo sustentável. In: C. Nunes-da-Cunha, M. T. F. Piedade, & W. J. Junk (Eds.), Classificação e delineamento das áreas úmidas brasileiras e de seus macrohabitats. pp. 13–76. Cuiabá: EdUFMT.

Keeley, J. E., & Fotheringham, C. J. 2000. Role of fire in regeneration from seed. In: M. Fenner (Ed), Seeds: The ecology of regeneration in plant communities. pp. 311–330. Wallingford: CABI Publishing.

Keeley, J. E., Pausas, J. G., Rundel, P. W., Bond, W. J., & Bradstock, R. A. 2011. Fire as an evolutionary pressure shaping plant traits. Trends in Plant Science, 16(8), 406–411. DOI: 10.1016/j.tplants.2011.04.002

Kwiatkowska-Falinska, A., Jankowska-Blaszczuk, M., & Jaroszewicz, B. 2014. Post-fire changes of soil seed banks. Polish Journal of Ecology, 62(3), 455–466. DOI: 10.3161/104.062.0307

Leal, E. C., Vieira, I. C. G., & Kato, M. S. A. 2006. Banco de sementes em sistemas de produção de agricultura com queima e sem queima no município de Marapanim, Pará. Boletim do Museu Paraense Emilio Goeldi - Ciências Naturais, 1(1), 19–29.

Ledru, M. P., Salgado-Labouriau, M. L., & Lorscheitter, M. L. 1998. Vegetation dynamics in southern and central Brazil during the last 10,000 yr B.P. Review of Paleobotany and Palynology, 99(2), 131–142. DOI: 10.1016/S0034-6667(97)00049-3

Legendre, P., & Legendre, L. 2012. Numerical Ecology. Amsterdam: Elsevier: p. 1006.

Lipoma, M. L., Funes, G., & Díaz, S. 2018. Fire effects on the soil seed bank and post-fire resilience of a semi-arid shrubland in central Argentina. Austral Ecology, 43, 46–55. DOI: 10.1111/aec.12533

López-Mariño, A., Luis-Calabuig, E., Fillat, F., & Bermúdez, F. F. 2000. Floristic composition of established vegetation and the soil seed bank in pasture communities under different traditional management regimes. Agriculture Ecosystems and Environment, 78, 273–282. DOI: 10.1016/S0167-8809(99)00137-1

Melo, A. C. G., Durigan, G., & Gorenstein, M. R. 2007. Efeito do fogo sobre o banco de sementes em faixa de borda de floresta estacional semidecidual, SP, Brasil. Acta Botanica Brasilica, 21(4), 927–934. DOI: 10.1590/S0102-33062007000400017

Miao, S., Edelstein, C., Carstenn. S., & Gu, B. 2010. Immediate ecological impacts of a prescribed fi re on a cattail-dominated wetland in Florida Everglades. Fundamental and Applied Limnology, 176(1), 29–41. DOI: 10.1127/1863-9135/2010/0176-0029

Miranda, C. S., Paranho Filho, Conceição, A., & Pott, A. 2018. Changes in vegetation cover of the Pantanal wetland detected by Vegetation Index: a strategy for conservation. Biota Neotropica, 18(1), e20160297. Retrieved on July 30, 2019, from http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032018000100202&lng=en&nrm=iso. DOI: 10.1590/1676-0611-bn-2016-0297

Nunes-da-Cunha, C., Junk, W. J., & Leitão-Filho, H. F. 2007. Woody vegetation in the Pantanal of Mato Grosso, Brazil: a preliminary tipology. Amazoniana, 19(3/4), 159–184.

Nunes-da-Cunha, C., & Junk, W. J. 2015. A classificação dos macrohabitats do Pantanal Matogrossense. In: C. Nunes-da-Cunha, M. T. F. Piedade, & W. J. Junk (Eds.), Classificação e delineamento das Áreas Úmidas brasileiras e de seus macrohabitats. pp. 77–126. Cuiabá: EdUFMT.

Oksanen, J., Blanchet, F. G., Friendly, M., Kindt, R., Legendre, P., McGlinn, D., & Wagner, H. 2018. Vegan: Community Ecology Package. Version 2.5-2. Retrieved from https://cran.r-project.org/web/packages/vegan/vegan.pdf

Parker, V.T., & Kelly, V. R. 1989. Seed banks in California chaparral and other Mediterranean climate shrublands. In: M. A. Leck, V. T. Parker, & R. L. Simpson (Eds.). Ecology of soil seed banks. pp. 231–256. San Diego: Academic Press.

Oliveira, P. C. 2009. Banco de sementes do solo de campos savânicos inundáveis no Pantanal de Mato Grosso. Master thesis. Instituto de Biociências da Universidade Federal de Mato Grosso. p. 79.

Oliveira, P. C., Torezan, J. M. D., & Nunes-da-Cunha, C. 2015. Effects of flooding on the spatial distribution of soil seed and spore banks of native grasslands of the Pantanal wetland. Acta Botanica Brasilica, 29, 400–407. DOI: 10.1590/0102-33062015abb0027

Parolin, P., Ferreira, L.V., & Junk, W. J. 2003. Germination characteristics and establishment of trees from Central Amazonian floodplains. Tropical Ecology, 44(2), 157–169.

Parolin, P., & Wittmann, F. 2010. Struggle in the flood: Tree responses to flooding stress in four tropical floodplain systems. AOB Plants (open access). DOI: 10.1093/aobpla/plq003

Pott, A., & Pott, V.J. 1994. Plantas do Pantanal. Brasília: Embrapa: p 320.

Pott, A., Oliveira, A. K. M., Damasceno-Junior, G. A., & Silva, J. S. V. 2011. Plant diversity of the Pantanal wetland. Brazilian Journal of Biology, 71 (1), 265–273. DOI: 10.1590/S1519-69842011000200005

Ratnam, J., Bond W. J., Fensham, R. J., Hoffmann, W. A., Archibald, S., Lehmann, C. E. R., Anderson, M. T., Higgins, S. I., & Sankaran, M. 2011. When is a ‘forest’ a savanna, and why does it matter? Global Ecology and Biogeography, 20, 653–660. DOI: 10.1111/j.1466-8238.2010.00634.x

Rebellato, L., & Nunes-da-Cunha, C. 2005. Efeito do “fluxo sazonal mínimo da inundação” sobre a composição e estrutura de um campo inundável no Pantanal de Poconé, MT, Brasil. Acta Botanica Brasilica, 19(4), 789–799. DOI: 10.1590/S0102-33062005000400015

Resende, E. K., & Galdino, S. 2001. O Pantanal está secando? Corumbá: Embrapa Pantanal. Retrieved on January, 2009, from http://www.cpap.embrapa.br/publicacoes

Ribeiro L. C., & Borghetti, F. 2014. Comparative effects of desiccation, heat shock and high temperatures on seed germination of savanna and forest tree species. Austral Ecology, 39, 267–278. DOI: 10.1111/aec.12076

Saboya, P., & Borghetti, F. 2012. Germination, initial growth, and biomass allocation in three native Cerrado species. Brazilian Journal of Botany, 35, 129–135. DOI: 10.1590/S0100-84042012000200002

Sarmiento, G., & Monasterio, M. 1983. Life forms and fenology. In: F. Bourliére (Ed.), Ecosystems of the Word: Tropical Savannas. pp. 79–108. Amsterdam: Elsevier.

Schessl, M. 1999. Floristic composition and structure of floodplain vegetation in northern Pantanal of Mato Grosso, Brasil. Phyton, 39(2), 303–336.

Silva, J. S. V., & Abdon, M. M. 1998. Delimitação do Pantanal brasileiro e suas sub-regiões. Pesquisa Agropecuária Brasileira, 33, 1703–1711.

Soriano, B. M. A., Daniel, O., & Santos, S. A. 2015. Eficiência de índices de risco de incêndios para o Pantanal sul-mato-grossense. Ciência Florestal, 25(4), 809–816. DOI: 10.5902/1980509820231

Souza, E. B., Ferreira, F. A., & Pott, A. 2016. Effects of flooding and its temporal variation on seedling recruitment from the soil seed bank of a Neotropical floodplain. Acta Botanica Brasilica, 30(4), 560–568. DOI: 10.1590/0102-33062016abb0202

Tesfaye, G., Teketay, D., Assefa, Y., & Fetene, M. 2004. The impact of fire on the soil seed bank and regeneration of Harenna Forest, Southeastern Ethiopia. Mountain Research and Development 24(4), 354–361. DOI: 10.1659/02764741(2004)024[0354:TIOFOT]2.0.CO;2

Thompson, K., Bakker, J., & Bekker, R. 1997. The soil seed banks of north west Europe: methodology, density and longevity. Cambridge: Cambridge University Press: p. 276.

Tuffi-Santos, L. D., Santos, I. C., Oliveira, C. H., Santos, M. V., Ferreira, F. A., & Queiroz, D.S. 2004. Levantamento fitossociológico em pastagens degradadas sob condições de várzea. Planta Daninha, 22(3), 343–349. DOI: 10.1590/S0100-83582004000300003

Veldman, J. W., Buisson, E., Durigan, G., Fernandes, G. W., Le Stradic, S., Mahy, G., Negreiros, D., Overbeck, G. E., Veldman, R. G., Zaloumis N. P., Putz, F. E., & Bond, W. J. 2015. Toward an old‐growth concept for grasslands, savannas, and woodlands. Frontiers in Ecology and the Environment, 13(3), 154–162. DOI: 10.1890/140270

Wantzen, K. M., Nunes-da-Cunha, C. N., Junk, W. J., Girard, P., Rossetto, O. C., Penha, J. M., Couto, E. G., Becker, M., Priante, G., Tomas, W. M., Santos, S. A., Marta, J., Domingos, I., Sonoda, F., Curvo, M., & Callil, C. 2008. Towards a sustainable management concept for ecosystem services of the Pantanal wetland. Ecohydrology & Hydrobiology, 8(2–4), 115–138. DOI: 10.2478/v10104-009-0009-9

Zirondi, H. L., Silveira, F. A. O., & Fidelis, A. T. 2019. Fire effects on seed germination: Heat shock and smoke on permeable vs impermeable seed coats. Flora, 253, 98–106. DOI: 10.1016/j.flora.2019.03.007

DOI: https://doi.org/10.4257/oeco.2019.2304.14


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