Evaluation of the addition of glycerol to Cupriavidus necator culture medium over Poly(3-hydroxybutyrate) production

Auteurs

  • Giannini Paszinick Apati
  • Maikon Kelbert
  • Bruna Regina Sombrio
  • Andrea Lima dos Santos Schneider
  • Michele Cristina Formolo Garcia
  • Agenor Furigo Junior
  • Ana Paula Testa Pezzin

Résumé

 

 Glycerol was used as a source of additional carbon in the production of Poly(3-hydroxybutyrate) (P(3HB)). The inverted sugar and glycerol concentrations and the temperature of the Cupriavidus necator culture medium were evaluated using a Central Composite Rotational Design (CCRD). The results showed that the increase in temperature and sugar concentration led to an increase in production and P(3HB) accumulation and when 15 g L-1 of glycerol was added better results were obtained, however these were not considered statistically significant. The best results were obtained at 38 °C and with 30 g L-1 of inverted sugar. Although not considered statistically significant, the addition of 15 g L-1 of glycerol increased the P(3HB) accumulation percentage by 15 %, thus in kinetic terms, greater productivity was obtained in 0.32 g L-1 h-1 polymer. Larger scale assays are being conducted to verify if the addition of glycerol improves the thermal and mechanical properties of the synthesized polymer.

Keywords: Poly(3-hydroxybutyrate), Cupriavidus necator, glycerol.

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Publiée

2018-08-15

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