The original description of Dasyatis colarensis was based solely on male specimens, when the females were until then unknown. Females were collected later in 2004 at the type locality, and they are described herein. An emended diagnosis for the species is also provided. Some characters only verified in females, as a slight notch on the medial portion of the snout, crenulate teeth crowns, and dermal denticles at the ventral side of the disc were identified as secondary sexual dimorphism. The maximum size of D. colarensis was also increased, for more than 2600mm of total length and 900mm of disc width.