GENETIC CONNECTIVITY OF MULLETS (MUGIL LIZA) BETWEEN RODRIGO DE FREITAS LAGOON AND A CONSERVATION UNIT IN STATE OF RIO DE JANEIRO, SOUTHEASTERN BRAZIL
DOI:
https://doi.org/10.4257/oeco.2021.2503.11Keywords:
, coastal ecosystem, gene flow, microsatellite.Abstract
We analyzed the genetic connectivity between mullets (Mugil liza) captured around the protected Natural Monument of Cagarras Islands (MoNa Cagarras) and inside Rodrigo de Freitas Lagoon, using microsatellite markers polymorphisms. Our data revealed the occurrence of 31 shared alleles (from 41 sampled), a high similarity in both allelic frequencies and genetic diversity and lack of differentiation between collection points (FST = 0.000, p > 0.05, STRUCTURE best estimative K = 1), results which, analyzed together, are strongly indicative of panmixia. We conclude that individuals collected inside the Rodrigo de Freitas Lagoon are genetically similar to those individuals collected around MoNa Cagarras. Given the importance of estuaries for the reproduction and development of individuals of M. liza, it is recommended that the Rodrigo de Freitas Lagoon to be managed in order to maintain genetic connectivity and diversity between the two ecosystems.
References
Amador, E. S. 1997. Baía de Guanabara e ecossistemas periféricos - Homem e Natureza. Rio de Janeiro: Reproarte Gráfica e Editora: p. 529.
Amorim, R. B. & Monteiro Neto, C. 2016. Marine protected area and the spatial distribution of the gill net fishery in Copacabana, Rio de Janeiro, RJ, Brazil. Brazilian Journal of Biology, 76 (1), 1–9. DOI: 10.1590/1519-6984.06614
Andreata, J. V., Manzano, F. V., Baptista, M. G. S., Teixeira, D. E., Oliveira, L. O. V., Longo, M. M., Freret, N. V. & Valois, A. S. 2002. Assembléia de peixes da lagoa Rodrigo de Freitas, Rio de Janeiro. Bioikos, 16 (1): 19–28.
Belkhir, K., Borsa, P., Chikhi, L., Raufaste, N. & Bonhomme, F. 2002. GENETIX 4.04, logiciel sous Windows TM pour la génétique des populations. Laboratoire Génome, Populations, Interactions, CNRS UMR 5000, Université de Montpellier II, Montpellier (France).
Bertoncini, A. A., Rangel, C. A., Chaves, L. C. T., Mendonça-Neto, J. P. & Monteiro Neto, C. 2013. Peixes Recifais do Monumento Natural das Ilhas Cagarras. In: Moraes, F., Bertoncini, A. & Aguiar, A. (Eds.), História, Pesquisa e Biodiversidade do Monumento Natural das Ilhas Cagarras. pp. 107–137. Rio de Janeiro: Museu Nacional.
Brasil, 2020. Plano de manejo do monumento natural do arquipélago das Ilhas Cagarras. Instituto Chico Mendes de Conservação da Biodiversidade (ICMBio). Brasília, DF.
Cuéllar-Pinzón, J., Presa, P., Hawkins, S. & Pita, A. 2016. Genetic markers in marine fisheries: Types, tasks and trends. Fisheries Research, 173, 194–205. DOI:10.1016/j.fishres.2015.10.019
Earl, D. & Von Holdt, B. 2012. STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conservation Genetics Resources, 4, 359–361. DOI: 10.1007/s12686-011-9548-7
Excoffier, L. & Lischer, H. E. L. 2010. Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources, 10, 564–567. DOI: 10.1111/j.1755-0998.2010.02847.x
Fortunato, R. C., González-Castro, M., Galán, A. R., Alonso, I. G., Kunert, C., Durà, V. B. & Volpedo, A. 2017. Identification of potential fish stocks and lifetime movement patterns of Mugil liza Valenciennes 1836 in the Southwestern Atlantic Ocean. Fisheries Research, 193, 164–172. DOI: 10.1016/j.fishres.2017.04.005
Garbin, T., Castello, J. P. & Kinas, P. G. 2014. Age, growth and mortality of the mullet Mugil liza in Brazil's southern and southeastern coastal regions. Fisheries Research, 49, 61–68. DOI: 10.1016/j.fishres.2013.09.008
Gonzalez, A. M., Paranhos, R. & Lutterbach, M. S. 2006. Heterotrophic bacteria abundances in Rodrigo de Freitas Lagoon (Rio de Janeiro, Brazil). Brazilian Journal of Microbiology, 37 (4), 428–433. DOI: 10.1590/S1517-83822006000400005
Goudet, J. 2001. FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Avaiable at: http://www2.unil.ch/popgen/softwares/fstat.htm.
Kasapidis, P., Silva, A., Zampicinini, G. & Magoulas, A. 2012. Evidence for microsatellite hitchhiking selection in European sardine (Sardina pilchardus) and implications in inferring stock structure. Scientia Marina, 76, 123–132. DOI: 10.3989/scimar.03366.29B
Mai, A. C. G., Miño, C. I., Marins, L. F., Monteiro-Neto, C., Miranda, L., Schwingel, P. R., Lemos, V. N., Gonzalez-Castro, M., Castello, J. P & Vieira, J. P. 2014. Microsatellite variation and genetic structuring in Mugil liza (Teleostei: Mugilidae) populations from Argentina and Brazil. Estuarine, Coastal and Shelf Science, 149, 80–86. DOI: 10.1016/j.ecss.2014.07.013.
Menezes, N. A., Oliveira, C. & Nirchio, M. 2010. An old taxonomic dilemma: the identity of the western south Atlantic Lebranche Mullet (Teleostei: Perciformes: Mugilidae). Zootaxa, 2519, 59–68. DOI: 10.5281/zenodo.196200
Miggiano, E., Lyons, R. E., Li, Y., Dierens, L. M., Crosetti, D. & Sola, L., 2005. Isolation and characterization of microsatellite loci in the striped mullet, Mugil cephalus. Molecular Ecology Resources, Primer Notes, 5, 323–326. DOI:10.1111/j.1471-8286.2005.00915.x
Miller, S. A., Dykes, D. D. & Polesky, H. F. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research, 16, 1215. DOI: 10.1093/nar/16.3.1215
Potter, I. C., Tweedley, J. R., Elliott, M. & Whitfield, A. K. 2013. The ways in which fish use estuaries: a refinement and expansion of the guild approach. Fish and Fisheries, 16 (2), 230–239. DOI: 10.1111/faf.12050
Pritchard, J. K., Stephens, M. & Donnelly, P. 2000. Inference of population structure using multilocus genotype data. Genetics, 155, 945-959.
Schuelke, M. 2000. An economic method for the fluorescent labeling of PCR fragments. Nature Biotechnology, 18, 233–234. DOI: 10.1038/72708
Shen, K. N., Chen, C. Y., Tzeng, W. N., Chen, J. D., Knibb, W. & Durand, J. D. 2010. Development and characterization of 13 GT/CA microsatellite loci in cosmopolitan flathead mullet Mugil cephalus. Molecular Ecology Resources, 10 (6), 1098–1105. DOI: 10.1007/s12686-011-9400-0
Siccha-Ramirez, R., Menezes, N. A., Nirchio, M., Foresti, F. & Oliveira, C. 2014. Molecular identification of mullet species of the Atlantic South Caribbean and South America and the phylogeographic analysis of Mugil liza. Reviews in Fisheries Science and Aquaculture, 22 (1), 86–96. DOI: 10.1080/10641262.2013.833583
Van Oosterhout, C., Hutchinson, W. F., Willis, D. P. M. & Shipley, P. 2004. Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes, 4, 535–538. DOI: 10.1111/j.1471-8286.2004.00684.x
Vieira, J. P., Garcia, A. M. & Grimm, A. M. 2008. Evidences of El Niño effects on the mullet fishery of the Patos Lagoon Estuary. Brazilian Archives of Biology and Technology, 51, 433–440. DOI: 10.1590/S1516-89132008000200025
Vieira, J. P. & Scalabrin, C. 1991. Migração reprodutiva da “Tainha” (Mugil platanus, Günther, 1880) no sul do Brasil. Atlântica, 13 (1), 131–141.
