HYDRODYNAMIC DIAMETER CONTROL IN THE SYNTHESIS OF CHITOSAN NANOPARTICLES: OPTIMIZATION BY EXPERIMENTAL DESIGN
DOI:
https://doi.org/10.55747/bjedis.v3i1.60159Keywords:
nanoparticles, chitosan, experimental design, hydrodynamic diameterAbstract
Background: Chitosan nanoparticles are an alternative vehicle for drug formulation and delivery. Objective: This study aims at the synthesis of chitosan/tripolyphosphate nanoparticles through an experimental design 32, to evaluate the ideal conditions for obtaining a reduced particle diameter with potential for encapsulation of biomolecules. Method: The nanoparticles were obtained by ionotropic gelation, in 11 experimental conditions. Statistical Analysis were carried out using the software Statistics 7, having as independent variable: the polymer/crosslinker mass ratio and the drip flow rate of the crosslinker. The hydrodynamic diameter of the nanoparticles was the response variable, which was characterized by dynamic light scattering. Results: For the optimized system the results show that the mass ratio between the reactants influences the particle size obtained, and the mass ratio of Chitosan/TPP between 2.25 - 2.50 is the most indicated for obtaining diameters between 20-50 nm. Conclusion: Considering the range of hydrodynamic diameters observed in this study, the nanoparticles are well-suited for utilization as active carriers, specifically targeting organs to enhance the effectiveness of therapies.
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We at this moment declare that the present paper is our original work and has not been previously considered, either in whole or in part, for publication elsewhere. Besides, we warrant the authors will not submit this paper for publication in any other journal. We also guarantee that this article is free of plagiarism and that any accusation of plagiarism will be the authors' sole responsibility. The undersigned transfer all copyrights to the present paper (including without limitation the right to publish the work in any and all forms) to BJEDIS, understanding that neglecting this agreement will submit the violator to undertake the legal actions provided in the Law on Copyright and Neighboring Rights (No. 9610 of February 19, 1998). Also, we, the authors, declare no conflict of interest. Finally, all funders were cited in the acknowledgments section.
