• Marcelo Magioli Universidade de São Paulo, Escola Superior de Agricultura “Luiz de Queiroz”, Departamento de Ciências Florestais, Laboratório de Ecologia, Manejo e Conservação da Fauna Silvestre (LEMaC), Av. Pádua Dias, 11, Piracicaba, SP, Brazil, CEP 13418-900.
  • Alex Augusto Abreu Bovo Universidade de São Paulo, Escola Superior de Agricultura “Luiz de Queiroz”, Departamento de Ciências Florestais, Laboratório de Ecologia, Manejo e Conservação da Fauna Silvestre (LEMaC), Av. Pádua Dias, 11, Piracicaba, SP, Brazil, CEP 13418-900.
  • Marcel Pieter Huijser Montana State University, Western Transportation Institute, PO Box 174250, Bozeman, MT, USA, 59717-4250.
  • Fernanda Delborgo Abra Universidade de São Paulo, Escola Superior de Agricultura “Luiz de Queiroz”, Departamento de Ciências Florestais, Laboratório de Ecologia, Manejo e Conservação da Fauna Silvestre (LEMaC), Av. Pádua Dias, 11, Piracicaba, SP, Brazil, CEP 13418-900.
  • Renata Alonso Miotto Universidade de São Paulo, Escola Superior de Agricultura “Luiz de Queiroz”, Departamento de Ciências Florestais, Laboratório de Ecologia, Manejo e Conservação da Fauna Silvestre (LEMaC), Av. Pádua Dias, 11, Piracicaba, SP, Brazil, CEP 13418-900.
  • Victor Hugo Vasconcellos Prado Andrade Universidade de São Paulo, Escola Superior de Agricultura “Luiz de Queiroz”, Departamento de Ciências Florestais, Laboratório de Ecologia, Manejo e Conservação da Fauna Silvestre (LEMaC), Av. Pádua Dias, 11, Piracicaba, SP, Brazil, CEP 13418-900.
  • Adriana Marques Nascimento Universidade de São Paulo, Escola Superior de Agricultura “Luiz de Queiroz”, Departamento de Ciências Florestais, Laboratório de Ecologia, Manejo e Conservação da Fauna Silvestre (LEMaC), Av. Pádua Dias, 11, Piracicaba, SP, Brazil, CEP 13418-900.
  • Maísa Ziviani Alves Martins Universidade de São Paulo, Escola Superior de Agricultura “Luiz de Queiroz”, Departamento de Ciências Florestais, Laboratório de Ecologia, Manejo e Conservação da Fauna Silvestre (LEMaC), Av. Pádua Dias, 11, Piracicaba, SP, Brazil, CEP 13418-900.
  • Katia Maria Paschoaletto Micchi de Barros Ferraz Universidade de São Paulo, Escola Superior de Agricultura “Luiz de Queiroz”, Departamento de Ciências Florestais, Laboratório de Ecologia, Manejo e Conservação da Fauna Silvestre (LEMaC), Av. Pádua Dias, 11, Piracicaba, SP, Brazil, CEP 13418-900.



human-modified landscapes, mammals, mitigation measures, roadkill


Short and narrow roads are generally overlooked when assessing road impacts on biodiversity. However, these roads bisect natural environments and may cause significant impacts on wildlife in local scale. Thus, we monitored roadkills along a short two-lane road (CPM road) in Southeastern Brazil and propose mitigation strategies to reduce wildlife mortality. We monitored roadkilled vertebrates along 5 km of CPM road from 2010 to 2016 and we also compiled data from previous studies along the same road. We conducted a hotspot analysis to identify CPM road areas with significant roadkill aggregation. We recorded 77 roadkilled vertebrates from 14 taxonomic groups along the CPM road. Mammals were the most frequently recorded group (91% of roadkills), which represented 56% of all medium- and large-sized mammal species known to occur in the study area. We identified three roadkill hotspots along the CPM road. Two of them were located at two stream crossings, where the road cut across the associated riparian forests, and the other was at a road section with water drainage from a pond, also connected to a riparian forest. These riparian forests are part of the remaining natural habitat that provides connectivity between the forest remnants in the landscape, and therefore, for wildlife. Our results showed that even short and narrow roads can have considerable roadkill, which may have severe effects for wildlife on a local scale. The results stress the need to carefully look at these types of roads and propose measures to reduce impacts. We propose the creation of safe crossing opportunities in the hotspot zones combined with wildlife fencing to keep the animals off the road and guide them towards the safe crossing opportunities.


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