DISRUPTION AND RECOVERY OF BACTERIAL COMMUNITY STRUCTURE OF AN ATLANTIC FOREST SOIL AFTER EXPOSURE TO GASOHOL

Authors

  • Janaína Fernandes Araújo Universidade Federal de Viçosa, Programa de Pós-Graduação em Microbiologia Agrícola, Microbiology Department, Laboratório de Biotecnologia e Biodiversidade para o Meio Ambiente – LBBMA. P.H. Rolfs St. CEP:36570-000. Viçosa, MG, Brazil
  • Rita de Cássia Rocha Fernandes Universidade Federal de Viçosa, Programa de Pós-Graduação em Microbiologia Agrícola, Microbiology Department, Laboratório de Biotecnologia e Biodiversidade para o Meio Ambiente – LBBMA. P.H. Rolfs St. CEP:36570-000. Viçosa, MG, Brazil
  • Edmo Montes Rodrigues Universidade Federal de Viçosa, Programa de Pós-Graduação em Microbiologia Agrícola, Microbiology Department, Laboratório de Biotecnologia e Biodiversidade para o Meio Ambiente – LBBMA. P.H. Rolfs St. CEP:36570-000. Viçosa, MG, Brazil
  • Marcos Rogério Tótola Universidade Federal de Viçosa, Programa de Pós-Graduação em Microbiologia Agrícola, Microbiology Department, Laboratório de Biotecnologia e Biodiversidade para o Meio Ambiente – LBBMA. P.H. Rolfs St. CEP:36570-000. Viçosa, MG, Brazil

DOI:

https://doi.org/10.4257/oeco.2019.2303.07

Keywords:

hydrocarbon bioremediation, microbial ecology, microbial succession, resilience, Tropical forest soil

Abstract

This article reports the effects of gasohol on the genetic of a bacterial community of a tropical Atlantic Forest soil. Hydrocarbon and ethanol biodegradation was accompanied by CO2 emission. Gasohol had an immediate impact on genetic structure of bacteria and on respiratory metabolism of soil microbial community. Cluster analysis of DGGE band pattern indicated a shift in the community structure between the fifth and fortieth days after contamination. At 60 days after contamination, the DGGE profile of the bacterial community in the contaminated soil was similar to that found in the non-contaminated control. Gasohol addition increased the respiratory rate of the soil, peaking at 3 days and returning to basal level at 15 days after contamination. We concluded that gasohol contamination causes a strong transient impact on soil microbial community structure that is completely reversed after a few days following contaminant removal. Secondary succession after contamination resulted in a bacterial community of identical genetic structure to that found before contamination. Our results point out to a high resilience of microbial community established in Atlantic Forest soil.

Author Biography

Rita de Cássia Rocha Fernandes, Universidade Federal de Viçosa, Programa de Pós-Graduação em Microbiologia Agrícola, Microbiology Department, Laboratório de Biotecnologia e Biodiversidade para o Meio Ambiente – LBBMA. P.H. Rolfs St. CEP:36570-000. Viçosa, MG, Brazil

Microbiology Department

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Published

2019-09-06

Issue

Vol. 23 No. 3 (2019): Spontaneous submissions

Section

Articles